Normally this pressure is 0.25 inches of water column, but may be lifted or reduced as the method involves. Isolators have to be periodically leak tested to make certain their integrity and prevent escape on the decontamination agent. Leak testing can be done by different methods which includes tension https://tailinscitech.wixsite.com/tailinscitech/post/applications-and-benefits-of-tailin-containment-isolator-for-handling-high-potency-materials