Next, we treated cells with different concentrations of NAD+. When additional for 24 h at eighty µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Prolonged Knowledge Fig. 1k,l). Additionally, 16 µM of NAD+ was adequate to reduce fork velocity by ~40% in HeLa cells https://gunnerthuqe.weblogco.com/31813979/a-secret-weapon-for-nad-and-its-effect-on-dna-repair